Product/Service

Semen Analysis Kit

Source: ENG Scientific, Inc.
This kit consists of a diluting fluid that renders spermatozoa immobile for easy chamber counting; a vitality stain that will easily differentiate between viable and non-viable spermatozoa; and a rapid stain and buffer solution requiring less than 60 seconds for the detection of pus cells (WBC's, RBC's, etc.).
Solution 1 - Semen Diluting Fluid
Solution 11A - 5% Aqueous Eosin
Solution 11B - 1% Aqueous Nigrosin
Solution 111A - Stain
Solution 111B - Buffer

This kit consists of a diluting fluid that renders spermatozoa immobile for easy chamber counting; a vitality stain that will easily differentiate between viable and non-viable spermatozoa; and a rapid stain and buffer solution requiring less than 60 seconds for the detection of pus cells (WBC's, RBC's, etc.).

RECOMMENDED PROCEDURE: Sperm Count: Draw semen up to 0.5 mark (WBC pipette). Dilute to the 11.0 mark with Sol. 1. Shake well. Charge hemacytometer and place in humidified chamber to avoid evaporation. Count the number of spermatazoa in 2 sq. mm and add 5 zeros to obtain the number per ml. Vitality Staining: Mix a drop of semen with 2 drops Sol. llA. Add 4 drops of Sol. IIB, mix gently and prepare smears. Fix by heat. Staining For Pus Cells, Red Cells, Spermatozoa Morphology, etc.: Smear a drop of semen on a clean glass slide. Place in Sol. 111A for 20 seconds, followed by Sol. 1llB for 20 seconds.

RESULTS: Total Count: 60,000,000 and higher. Vitality Stain: Dead, non-viable spermatozoa will stain red, while viable spermatozoa will be unstained (The Blom Technique). Stain For Pus Cells, etc.: Similar to Wright Stain.

CONTENTS: Sodium Bicarbonate, Formalin (Neutral), Eosin, Nigrosin, Wright Stain, Methyl Alcohol, Buffers
These solutions are made from certified dyes (when applicable). For in vitro diagnostic use only.

ENG Scientific, Inc., 82 Industrial East, Clifton, NJ 07012. Tel: 973-472-7200; Fax: 973-472-9460.