Gram Stain Kit For Differential Staining Of Bacteria
Solution 11- Grams Iodine
Solution III - 70% Alcohol
Solution IIIA - 95% Alcohol
Solution I1IB - Acetone-Alcohol
Solution IV - Safranin
Solution IVA - Basic Fuchsin
Solution IVB - Carbol Fuchsin
AVAILABLE IN VARIOUS COUNTERSTAINS AS FOLLOWS:
Safranin - Routinely used for gram negative bacteria.
Basic Fuchsin - More intense overall staining to clearly identify weak gram-negative bacteria and also the easy detection of Bordatella.
Carbol Fuchsin - A counterstain of greater overall intensity to easily identify Legionella.
Also available with various decolorizers to suit individual preference of technique. Convenient flip-top cap for 250 ml and 950 ml sizes.
RECOMMENDED PROCEDURE: Cover fixed smear with Sol. I for 1 minute and rinse gently with distilled water. Apply Sol. 11 for 1 minute and rinse gently with distilled water. Decolorize with Sol. III, Sol. IIIA, or Sol. 111B until the purple color no longer runs off the slide, (from 2 - 20 seconds depending upon decolorizer and thickness of smear) and rinse with distilled water. Counterstain with Sol. IV, Sol. IVA or Sol. IVB for 1 minute and rinse with distilled water. Allow to dry and examine under oil immersion.
NOTE: For a faster decolorizer, use Sol. I11B - Acetone-Alcohol. For a more intense counterstain, we recommend Sol. IVA - Basic Fuchsin or Sol. IVB - Carbol Fuchsin.
RESULTS: Gram-positive bacteria stain purple to blue black. Gram-negative bacteria stain pink to red.
CONTENTS: Crystal Violet, Ethyl Alcohol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol
These solutions are made from certified dyes (when applicable). For in vitro diagnostic use only.
ENG Scientific, Inc., 82 Industrial East, Clifton, NJ 07012. Tel: 973-472-7200; Fax: 973-472-9460.