Fetal Hemoglobin - Differential Staining Kit

This product has been developed to employ a new modified Kleihauer procedure for fetal hemoglobin that does not require heat as in the old modified Kleihauer technique. This test can now be performed at room temperature. We recommend the use of hematoxylin for staining the WBC's so as to avoid misidentification of eosin-positive cells. This is done with the new Kleihauer technique rather than the new Clayton technique.

These procedures are based upon the resistance of fetal hemoglobin to acid conditions. Fixation of the smear allows hemoglobin to precipitate within the RBC. The Citric acid/Phosphate buffer solution eludes the adult hemoglobin. Fetal hemoglobin remains precipitated and stains bright red with eosin, making for easy differentiation. In the new and old Kleihauer procedure, WBC's are counterstained with hematoxylin to avoid misidentification as eosin-positive cells.

RECOMMENDED PROCEDURE: Prepare thin (monolayer) blood films of capillary or fresh EDTA-anticoagulated blood and fix in Sol. I for 5 minutes. Rinse with distilled water and allow to dry. Place slide in Sol. 11 or flood to elude 5 - 10 minutes. Rinse with distilled water. Stain with Sol. III for 1 - 4 minutes and rinse with distilled water. Stain with Sol. IV for 2 - 6 minutes. Rinse again in distilled water and allow time to dry.

RESULTS: RBC's containing Hb F stain brilliantly with eosin, while those without Hb F appear as colorless "ghosts". The hematoxylin stains the nuclei of the WBC's blue to prevent misinterpretation as eosin-positive cells.

CONTENTS: Citric Acid, Disodium Phosphate, Hematoxylin, Sodium Iodate, Potassium Alum, Eosin.

These solutions are made from certified dyes (when applicable). For in vitro diagnostic use only.

ENG Scientific, Inc., 82 Industrial East, Clifton, NJ 07012. Tel: 973-472-7200; Fax: 973-472-9460.